The antibody BU20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.

PRODUCT DETAILS

Clone: BU20A

Specificity: BrdU

Format: PE

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Ashby J, et al. 1999. Environ Health Perspect 107:819-22.
2. Soames AR, et al. 1994. J Histochem Cytochem 42:939-44.
3. Magaud JP, et al. 1989. J Histochem Cytochem 37:1517-27.
4. O'Driscoll M, et al. 2008. Bone Marrow Transplant 41:983-9.
5. Pajalunga D, et al. 2007. J Cell Biol 176:807-18.
6. Muskhelishvili L, et al. 2003. J Histochem Cytochem 51:1681-8.
7. Lagares A, et al. 2007. J Neurosci 27:7939-53.
8. Shih CH, et al. 2005. Am J Physiol Cell Physiol 289:C757-65.
9. Martín CA, et al. 2005. Biocell 29:33-8.
10. Yatera K, et al. 2008. Am J Physiol Heart Circ Physiol 294:H944-53.
11. Magaud JP, et al. 1989. J Histochem Cytochem 37:1517-27.

For research use only. Not intended for use in diagnostic procedures.