Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1), PYD, and CARD domain containing protein (PYCARD), is a 21.6 kD pro-apoptotic protein that contains a N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm. However, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocates from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Also, the AIM2/ASC complex can act as a novel caspase-8 activation platform. Recent studes has shown that ASC reduces the phosphorylation of IKKα/β phosphorylation and inhibits NF-κB activity in primary melanoma. However, in metastatic melanoma through, it has the opposite function enhancing NF-κB activity and the secretion of IL-1β.

PRODUCT DETAILS

Clone: O93E9

Antibody Type: Monoclonal

Reactivity: Human

Format: ASC

Host: Mouse

Isotype: IgG1, κ

Applications: ICFC - Quality tested

Regulatory Status: RUO

Data Sheets: TDS | SDS

Note to Purchaser: This product is manufactured and supplied by BioLegend, Inc. under an agreement between BioLegend, Inc. and Cytek Biosciences, Inc. Information related to this product is provided by BioLegend, Inc.