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Tonbo Biosciences PE Annexin V Apoptosis Detection Kit with 7-AAD contains the reagents needed identify apoptotic or necrotic cells by flow cytometry.

• PE Annexin V (Cat. No. 50-6409-T100): 100 tests. Annexin V belongs to the annexin family of calcium-dependent phospholipid binding proteins and has a high affinity for phosphatidylserine (PS). One of the characteristic features of early stage apoptosis is the translocation of PS from the inner to the outer leaflet of the plasma membrane, exposing it to the extracellular environment where it can be detected. Fluorochrome conjugated Annexin V can serve as a sensitive detection probe for this hallmark of early stage apoptotic change.
• Annexin V Binding Buffer (1X) (Cat. No. TNB-5000-L050): 50 mL. Annexin V Binding Buffer is formulated to facilitate the binding of Annexin V conjugates to phosphatidylserine for subsequent analysis by flow cytometry. This buffer is supplied as a 1X solution and requires no manipulation prior to use.li>
• 7-AAD (Cat. No. 13-6993-T200): 200 tests. 7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data.