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Mouse bone marrow cells heat killed at 65°C for 10 minutes and then mixed with live mouse bone marrow cells. Cells were then stained with Ghost Violet 540. Viable gate is indicated.

Ghost Dye™ Violet 540

SKU 13-0879-T100
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Ghost Dye™ Violet 540 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 540 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
Recent Citations:

Tapanes SA, Arizanovska D, Díaz MM, Folorunso OO, Harvey T, Brown SE, Radzishevsky I, Close LN, Jagid JR, Graciolli Cordeiro J, Wolosker H, Balu DT, Liebl DJ. Inhibition of glial D-serine release rescues synaptic damage after brain injury. Glia. 2022 Feb 23. doi: 10.1002/glia.24161. Epub ahead of print. PMID: 35195906.

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Name Ghost Dye™ Violet 540
Cat. No. 13-0879
Protocol Download Protocol
Excitation Laser Violet (405 nm)
Emission (nm) 537
Formulation 1 µL/test in DMSO
Application Flow Cytometry
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