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Mouse splenocytes were stimulated overnight with PMA and ionomycin and stained with Ghost UV 450. Viable gate is indicated.

Ghost Dye™ UV 450

SKU 13-0868-T100
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Ghost Dye™ UV 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ UV 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.


Recent Publications:

Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021 Apr 13;35(2):108966. doi: 10.1016/j.celrep.2021.108966. PMID: 33852868.

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Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093
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Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077
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Name Ghost Dye™ UV 450
Cat. No. 13-0868
Protocol Download Protocol
Excitation Laser UV (355 nm)
Emission (nm) 450
Formulation 1 µL/test in DMSO
Application Flow Cytometry
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