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Mouse bone marrow cells were heat killed at 65°C for 10 minutes and then mixed with live mouse bone marrow cells. Cells were then stained with Ghost Dye™ Blue 516. Viable gate is indicated.

Ghost Dye™ Blue 516

SKU 13-0867-T100
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Ghost Dye™ Blue 516 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Blue 516 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
Name Ghost Dye™ Blue 516
Cat. No. 13-0867
Protocol Download Protocol
Excitation Laser 488 nm
Emission (nm) 516
Formulation 1 µL/test in DMSO
Application Flow Cytometry
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