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Foxp3 / Transcription Factor Staining Buffer Kit

Foxp3 / Transcription Factor Staining Buffer Kit

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Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. Included are the following components, to be used during staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma.

Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) (Cat. No. TNB-1020-L050): 50 mL. A concentrated solution which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provide best results during intracellular staining of transcription factors using fluorescently conjugated antibodies.

Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (Cat. No. TNB-1022-L160): 160 mL. Intended for use as a diluent for Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)

Flow Cytometry Perm Buffer (10X) (Cat. No. TNB-1213-L150): 150 mL. Provided as a concentrate which, when diluted with distilled water to a 1X solution, provide best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps.


Recent Publications:


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Chait M, Yilmaz MM, Shakil S, Ku AW, Dogra P, Connors TJ, Szabo PA, Gray JI, Wells SB, Kubota M, Matsumoto R, Poon MM, Snyder ME, Baldwin MR, Sims PA, Saqi A, Farber DL, Weisberg SP. Immune and epithelial determinants of age-related risk and alveolar injury in fatal COVID-19. JCI Insight. 2022 Apr 21:e157608. doi: 10.1172/jci.insight.157608. Epub ahead of print. PMID: 35446789.

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Núñez-Ruiz A, Sánchez-Brena F, López-Pacheco C, Acevedo-Domínguez NA, Soldevila G. Obesity modulates the immune macroenvironment associated with breast cancer development. PLoS One. 2022 Apr 26;17(4):e0266827. doi: 10.1371/journal.pone.0266827. PMID: 35472214; PMCID: PMC9041840.

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Tsukimoto S, Hakata Y, Tsuji-Kawahara S, Enya T, Tsukamoto T, Mizuno S, Takahashi S, Nakao S, Miyazawa M. Distinctive High Expression of Antiretroviral APOBEC3 Protein in Mouse Germinal Center B Cells. Viruses. 2022 Apr 17;14(4):832. doi: 10.3390/v14040832. PMID: 35458563

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Nixon BG, Chou C, Krishna C, Dadi S, Michel AO, Cornish AE, Kansler ER, Do MH, Wang X, Capistrano KJ, Rudensky AY, Leslie CS, Li MO. Cytotoxic granzyme C-expressing ILC1s contribute to antitumor immunity and neonatal autoimmunity. Sci Immunol. 2022 Apr 8;7(70):eabi8642. doi: 10.1126/sciimmunol.abi8642. Epub 2022 Apr 8. PMID: 35394814.

Burrack AL, Schmiechen ZC, Patterson MT, Miller EA, Spartz EJ, Rollins MR, Raynor JF, Mitchell JS, Kaisho T, Fife BT, Stromnes IM. Distinct myeloid antigen-presenting cells dictate differential fates of tumor-specific CD8+ T cells in pancreatic cancer. JCI Insight. 2022 Apr 8;7(7):e151593. doi: 10.1172/jci.insight.151593. PMID: 35393950.

Rana M, Kansal R, Chaib M, Teng B, Morrrison M, Hayes DN, Stanfill AG, Shibata D, Carson JA, Makowski L, Glazer ES. The pancreatic cancer immune tumor microenvironment is negatively remodeled by gemcitabine while TGF-β receptor plus dual checkpoint inhibition maintains antitumor immune cells. Mol Carcinog. 2022 Mar 23. doi: 10.1002/mc.23401. Epub ahead of print. PMID: 35319799.

Teghanemt A, Pulipati P, Misel-Wuchter K, Day K, Yorek MS, Yi R, Keen HL, Au C, Maretzky T, Gurung P, Littman DR, Issuree PD. CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element. Nat Commun. 2022 Mar 18;13(1):1477. doi: 10.1038/s41467-022-28914-4. PMID: 35304452.

Hernández-García E, Cueto FJ, Cook ECL, Redondo-Urzainqui A, Charro-Zanca S, Robles-Vera I, Conde-Garrosa R, Nikolić I, Sabio G, Sancho D, Iborra S. Conventional type 1 dendritic cells protect against age-related adipose tissue dysfunction and obesity. Cell Mol Immunol. 2022 Jan 4. doi: 10.1038/s41423-021-00812-7. Epub ahead of print. PMID: 34983945.

Wilfahrt D, Philips RL, Lama J, Kizerwetter M, Shapiro MJ, McCue SA, Kennedy MM, Rajcula MJ, Zeng H, Shapiro VS. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. Elife. 2021 Dec 2;10:e70978. doi: 10.7554/eLife.70978. PMID: 34854376; PMCID: PMC8639145.

Poon MML, Rybkina K, Kato Y, Kubota M, Matsumoto R, Bloom NI, Zhang Z, Hastie KM, Grifoni A, Weiskopf D, Wells SB, Ural BB, Lam N, Szabo PA, Dogra P, Lee YS, Gray JI, Bradley MC, Brusko MA, Brusko TM, Saphire EO, Connors TJ, Sette A, Crotty S, Farber DL. SARS-CoV-2 infection generates tissue-localized immunological memory in humans. Sci Immunol. 2021 Oct 7:eabl9105. doi: 10.1126/sciimmunol.abl9105. PMID: 34618554.

Yoast RE, Emrich SM, Zhang X, Xin P, Arige V, Pathak T, Benson JC, Johnson MT, Abdelnaby AE, Lakomski N, Hempel N, Han JM, Dupont G, Yule DI, Sneyd J, Trebak M. The Mitochondrial Ca2+ Uniporter is a Central Regulator of Interorganellar Ca2+ Transfer and NFAT Activation. J Biol Chem. 2021 Sep 6:101174. doi: 10.1016/j.jbc.2021.101174. PMID: 34499925.

Lei Y, Guerra Martinez C, Torres-Odio S, et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv. 2021 May 26;7(22):eabe7548. doi: 10.1126/sciadv.abe7548. PMID: 34039599.

Kim DI, Cho YB, Lim Y, Hong SH, Hahm B, Lee SM, Kang SC, Seo YJ. Chios mastic gum inhibits influenza A virus replication and viral pathogenicity. J Gen Virol. 2021 Jan 8. doi: 10.1099/jgv.0.001550. PMID: 33416468.

Kumar P, Arbieva ZH, Maienschein-Cline M, Ganesh BB, Ramasamy S, Prabhakar BS. Induction of Antigen-Independent Proliferation of Regulatory T-Cells by TNF Superfamily Ligands OX40L and GITRL. Methods Mol Biol. 2021;2248:63-71. doi: 10.1007/978-1-0716-1130-2_4. PMID: 33185867.

Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.

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Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.

Yashiro T, Yura S, Tobita A, Toyoda Y, Kasakura K, Nishiyama C. Pterostilbene reduces colonic inflammation by suppressing dendritic cell activation and promoting regulatory T cell development. FASEB J. 2020 Sep 22. doi: 10.1096/fj.202001502R. PMID: 32964554.

Semionatto IF, Palameta S, Toscaro JM, Manrique-Rincón AJ, Ruas LP, Paes Leme AF, Bajgelman MC. Extracellular vesicles produced by immunomodulatory cells harboring OX40 ligand and 4-1BB ligand enhance antitumor immunity. Sci Rep. 2020 Sep 16;10(1):15160. doi: 10.1038/s41598-020-72122-3. PMID: 32939048.

Yagi Y, Kuwahara M, Suzuki J, Imai Y, Yamashita M. Glycolysis and subsequent mevalonate biosynthesis play an important role in Th2 cell differentiation. 2020 Aug 12. Biochem Biophys Res Commun. 2020;S0006-291X(20)31554-0. doi:10.1016/j.bbrc.2020.08.009.

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Conor N. Gruber, Jorg J.A. Calis, Sofija Buta, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity, 2020 Aug 3.

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Long Y, Zhao X, Liu C, Xia C, Liu C. Activated inducible co-stimulator-positive (ICOS+ ) programmed death 1-positive (PD-1+ ) Tfh cells indicate disease activity and severity in ulcerative colitis patients [published online ahead of print, 2020 Jul 4]. Clin Exp Immunol. 2020;10.1111/cei.13485. doi:10.1111/cei.13485.

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J. Michael Stolley, Timothy S. Johnston, Andrew G. Soerens, Lalit K. Beura, Pamela C. Rosato, Vineet Joag, Sathi P. Wijeyesinghe, Ryan A. Langlois, Kevin C. Osum, Jason S. Mitchell, David Masopust; Retrograde migration supplies resident memory T cells to lung-draining LN after influenza infection. J Exp Med3 June 2020; 217 (8): e20192197. doi:

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IL-6 Deficiency Exacerbates Allergic Asthma and Abrogates the Protective Effect of Allergic Inflammation against Streptococcus pneumoniae Pathogenesis. Taylor Schmit, Sumit Ghosh, Ram Kumar Mathur, Tyler Barnhardt, Ganesh Ambigapathy, Min Wu, Colin Combs, M. Nadeem Khan. The Journal of Immunology June 15, 2020, ji1900755; DOI: 10.4049/jimmunol.1900755.

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Correlation of the levels of DNA-binding inhibitor Id3 and regulatory T cells with SLE disease severity. ChenLiu. SenYu. RongJin. YanLong. SongsongLu. YingSong. XiuyuanSun. Xiao-HongSun. YuZhang. J Autoimmun. 2020 Jun 11;102498. doi: 10.1016/j.jaut.2020.102498.

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Franco da Cunha F, Andrade-Oliveira V, Candido de Almeida D, Borges da Silva T, Naffah de Souza Breda C, Costa Cruz M, Faquim-Mauro EL, Antonio Cenedeze M, Ioshie Hiyane M, Pacheco-Silva A, Aparecida Cavinato R, Torrecilhas AC, Olsen Saraiva Câmara N. Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059

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Woolsey C, Jankeel A, Matassov D, et al. Immune correlates of postexposure vaccine protection against Marburg virus. Sci Rep. 2020;10(1):3071. Published 2020 Feb 20. doi:10.1038/s41598-020-59976-3.

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Laura S. Angelo, Lynn H. Bimler, Rana Nikzad, Kevin Aviles-Padilla, and Silke Paust. CXCR6+ NK Cells in Human Fetal Liver and Spleen Possess Unique Phenotypic and Functional Capabilities. Front Immunol. 2019 Mar 19;10:469. doi: 10.3389/fimmu.2019.00469

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Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

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Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.

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Karo JM, Schatz DG and Sun JC. 2014. Cell 159(1): 94-107. (Flow Cytometry)

Lambracht-Washington D and Rosenberg RN. 2015. Neurobiol Aging. 36(3): 1274-81. (Flow Cytometry)

Luo CT, Liao W, Dadi S, Toure A and Li MO. 2016. Nature. 529(7587): 532-536. (Flow Cytometry)

Name Foxp3 / Transcription Factor Staining Buffer Kit
Cat. No. TNB-0607
Protocol Foxp3 / Transcription Factor (Nuclear) Staining Protocol
Application Flow Cytometry
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