This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.

PRODUCT DETAILS

Clone: RTG2B1-2

Specificity: Isotype Control

Format: FITC

Isotype: Rat IgG2b

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

REFERENCES

1. Chen L, et al. 2006. J Immunol 177:2373-83.
2. Mor A, et al. 2007. Arterioscler Thromb Vasc Biol 27:893-900.
3. Koduru S, et al. 2012. Blood 119:2302-9.

For research use only. Not intended for use in diagnostic procedures.