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C57Bl/6 splenocytes were stimulated with PMA and Ionomycin (right panel) or unstimulated (left panel) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with 0.125 ug FITC Anti-Mouse IFN gamma (35-7311).

FITC Anti-Mouse IFN gamma (XMG1.2)

SKU 35-7311-U025
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The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).

The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of ≥≥IFN-g in a variety of assays (use format suitable for functional assays).

Recent Publications:

Chen D, Duan Z, Zhou W, et al. Japanese encephalitis virus-primed CD8+ T cells prevent antibody-dependent enhancement of Zika virus pathogenesis. J Exp Med. 2020;217(9):e20192152. doi:10.1084/jem.20192152.

Wen J, Wang YT, Valentine KM, Dos Santos Alves RP, Xu Z, Regla-Nava JA, Ngono AE, Young MP, Ferreira LCS4, Shresta S. CD4+T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection. Cell Rep. 2020 Apr 28;31(4):107566. doi: 10.1016/j.celrep.2020.107566.

Blagih J, Zani F, Chakravarty P, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30(2):481-496.e6. doi:10.1016/j.celrep.2019.12.028.

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584

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Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.

Zellweger RM, Tang WW, Eddy WE, King K, Sanchez MC and Shresta S. 2015. J Virol. 89(12): 6494-6505. (Flow Cytometry)

Name FITC Anti-Mouse IFN gamma (XMG1.2)
Cat. No. 35-7311
Alternative Names IFN-g, IFNg, Interferon-g, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Gene ID 15978
Clone XMG1.2
Isotype Rat IgG1, kappa
Reactivity Mouse
Format FITC
Application Flow Cytometry
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