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C57Bl/6 splenocytes were stained with 0.5 ug FITC Anti-Mouse CD3 (35-0032) (solid line) or 0.5 ug FITC Rat IgG2b isotype control (dashed line).

FITC Anti-Mouse CD3 (17A2)

SKU 35-0032-U025
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The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. Journal of Biological Chemistry. 287: 13324-13335).

Recent Citations:

Alam MM, Yang D, Li XQ, Liu J, Back TC, Trivett A, Karim B, Barbut D, Zasloff M, Oppenheim JJ. Alpha synuclein, the culprit in Parkinson disease, is required for normal immune function. Cell Rep. 2022 Jan 11;38(2):110090. doi: 10.1016/j.celrep.2021.110090. PMID: 35021075.

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Rappe JCF, Finsterbusch K, Crotta S, Mack M, Priestnall SL, Wack A. A TLR7 antagonist restricts interferon-dependent and -independent immunopathology in a mouse model of severe influenza. J Exp Med. 2021 Nov 1;218(11):e20201631. doi: 10.1084/jem.20201631. Epub 2021 Sep 2. PMID: 34473195.

» View on PubMed

Name FITC Anti-Mouse CD3 (17A2)
Cat. No. 35-0032
Clone 17A2
Isotype Rat IgG2b, κ
Reactivity Mouse
Format FITC
Application Flow Cytometry
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