The mouse monoclonal antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

PRODUCT DETAILS

Clone: 4H10

Specificity: IgE

Reactivity: Human

Format: FITC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Franklin EC. 1975. Acta Endocrinol Suppl (Copenh) 194:77-95.
2. Fuller JM, et al. 1975. Clin Chem 21:667-71.
3. Balogh Z, et al. 1980. Ann Rheum Dis 39:129-34.
4. Gould HJ, et al. 2000. Br Med Bull 56:908-24.
5. Brinkmann V, et al. 1993. Cell Immunol 152:323-32.
6. Eberlein B, et al. 2014. Cytometry B Clin Cytom.

For research use only. Not intended for use in diagnostic procedures.