The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.

PRODUCT DETAILS

Clone: LT95

Specificity: CD95 (FAS)

Reactivity: Human

Format: FITC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Brumatti G, et al. 2008. Exp Cell Res 314:554-63.
2. Guo Z, et al. 2005. Blood 106:2033-41.
3. Park DR, et al. 2003. J Immunol 170:6209-16.
4. Scaffidi C, et al. 1998. EMBO J 17:1675-87.
5. Drosopoulos KG, et al. 2005. J Biol Chem 280:22856-67.

For research use only. Not intended for use in diagnostic procedures.