The mouse monoclonal antibody clone HM47 binds to human CD79a, an intracellular component of the B-cell receptor (BCR) complex. CD79a (also known as mb-1 or Igα) is a 47 kDa type I transmembrane signaling molecule expressed in the cytoplasm and membrane of B-lineage cells from the earliest recognizable B-cell precursors through mature B cells, and is retained in most B-cell neoplasms. CD79a forms a heterodimer with CD79b to mediate surface immunoglobulin (sIg) transport and signal transduction via ITAM-dependent pathways. Clone HM47 recognizes an intracellular epitope of CD79a and is suitable for flow cytometry following appropriate fixation/permeabilization.

PRODUCT DETAILS

Clone: HM47

Specificity: CD79a

Reactivity: Human, Mouse

Format: FITC

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Mason DY, Cordell JL, Brown MH, et al. 1995. J Pathol. 175:151–160.
2. van Dongen JJM, Lhermitte L, Böttcher S, et al. 2012. Cytometry A. 81A:614–636.
3. Bene MC, Nebe T, Bettelheim P, et al. 1995. Leukemia. 9:1783–1786.

For research use only. Not intended for use in diagnostic procedures.