The mouse monoclonal antibody clone 124-1D1 binds to human CD7, also known as gp40 or Leu-9. CD7 is a 40 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is highly expressed on thymocytes and mature T cells and is also present on natural killer (NK) cells, hematopoietic progenitor cells, and a minor subset of monocytes. CD7 functions primarily as a costimulatory receptor. Engagement of CD7—including interaction with its characterized ligand SECTM1/K12—can enhance T-cell activation and proliferation and support NK-cell interferon-gamma secretion. CD7-mediated signaling involves PI3K-associated intracellular pathways. Clone 124-1D1 specifically recognizes human CD7 and is suitable for the identification and analysis of CD7-expressing lymphoid populations in flow cytometry and related immunoassays.

PRODUCT DETAILS

Clone: 124-1D1

Specificity: CD7

Reactivity: Human

Format: FITC

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. van Dongen JJM, Lhermitte L, Böttcher S, et al. 2012. Cytometry A. 81A: 614–636.
2. Bene MC, Nebe T, Bettelheim P, et al. 1995. Leukemia. 9(10):1783–1786.
3. Orfao A, Schmitz G, Brando B, et al. 1999. Clin Chem Lab Med. 37(11–12):983–990.

For research use only. Not intended for use in diagnostic procedures.