The antibody AK-4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

PRODUCT DETAILS

Clone: AK-4

Specificity: CD62P (P-Selectin)

Reactivity: Human, Non-Human Primate

Format: FITC

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Harakawa N, et al. 2007. Int Immunol 19:321-9.
2. Martinez M, et al. 2005. J Biol Chem 280:5378-90.
3. Ramachandran V, et al. 2004. Proc Natl Acad Sci U S A 101:13519-24.
4. Ramachandran V, et al. 2001. Proc Natl Acad Sci U S A 98:10166-71.
5. Ludwig RJ, et al. 2004. J Invest Dermatol 122:830-6.
6. Kowalska MA, et al. 1999. Br J Haematol 104:220-9.
7. Holme PA, et al. 1998. FASEB J 12:79-89.
8. Dunlop LC, et al. 1992. J Exp Med 175:1147-50.
9. Sato Y, et al. 2005. Blood 106:428-35.

For research use only. Not intended for use in diagnostic procedures.