The mouse monoclonal antibody clone MEM-63 binds to CD58, a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily, also known as lymphocyte function-associated antigen 3 (LFA-3). Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on both antigen presenting cells and target cells enhances T cell recognition via the interaction with CD2 on the T cell surface.

PRODUCT DETAILS

Clone: MEM-63

Specificity: CD58

Reactivity: Human

Format: FITC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223-252.
2. Dustin ML, et al. 1987. Nature 329:846-848.
3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861-1871.
4. Sanders ME, et al. 1988. J. Immunol. 140:1401-1407.
5. Song S, et al. 2021. Frontiers in Immunology. 12: Article 705140.

For research use only. Not intended for use in diagnostic procedures.