The mouse monoclonal antibody 4C8 recognizes CD52, a GPI-anchored glycopeptide expressed on the surface of lymphocytes, macrophages, epithelial cells of male reproductive tract, and mature sperms.

PRODUCT DETAILS

Clone: 4C8

Specificity: CD52

Reactivity: Human

Format: FITC

Isotype: Mouse IgG3

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Schröter S, et al. 1999. J Biol Chem 274:29862-73.
2. Treumann A, et al. 1995. J Biol Chem 270:6088-99.
3. Rowan WC, et al. 1995. Int Immunol 7:69-77.
4. Koyama K, et al. 2007. Soc Reprod Fertil Suppl 63:103-10.
5. Domagała A, et al. 2001. Med Sci Monit 7:325-31.
6. Masuyama J, et al. 2002. J Immunol 169:3710-6.
7. Masuyama J, et al. 1999. J Exp Med 189:979-90.
8. Toh BH, et al. 2013. Cell Mol Immunol 10:379-82.
9. Watanabe T, et al. 2006. Clin Immunol 120:247-59.

For research use only. Not intended for use in diagnostic procedures.