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Human peripheral blood lymphocytes were stained with 5 uL (1 ug) Anti-Human CD45RA FITC (35-0458) (solid line) or 1.0 ug FITC Mouse IgG2b isotype control (dashed line).

FITC Anti-Human CD45RA (HI100)

SKU 35-0458-T025
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The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB and CD45RO.

The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.

Recent Publications:

Tomellini E, Fares I, Lehnertz B, et al. Integrin-α3 Is a Functional Marker of Ex Vivo Expanded Human Long-Term Hematopoietic Stem Cells. Cell Rep. 2019;28(4):1063-1073.e5. doi:10.1016/j.celrep.2019.06.084.

Ryotokuji K, Nakajima T, Ishimaru K, Ozaki-Shimada A, Kihara K, Namiki Y and Otani S. 2015. Laser Therapy. 24(3): 179-188. (Flow Cytometry)

Name FITC Anti-Human CD45RA (HI100)
Cat. No. 35-0458
Gene ID 5788
Clone HI100
Isotype Mouse IgG2b, kappa
Reactivity Human
Cross Reactivity Chimpanzee
Format FITC
Application Flow Cytometry
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