The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

PRODUCT DETAILS

Clone: S-HCL-1

Specificity: CD22

Reactivity: Human

Format: FITC

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Walker JA, et al. 2008. Immunology 123:314-25.
2. Tateno H, et al. 2007. Mol Cell Biol 27:5699-710.
3. Tedder TF, et al. 2005. Adv Immunol 88:1-50.
4. James SE, et al. 2008. J Immunol 180:7028-38.
5. Huang J, et al. 2005. Am J Clin Pathol 123:826-32.
6. Foon KA, et al. 1986. Blood 68:1-31.

For research use only. Not intended for use in diagnostic procedures.