The antibody MEM-154 reacts with an extracellular epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes, and it can be used for mapping CD16-158V/F polymorphism on NK cells, as it requires presence of V at amino acid 158.

PRODUCT DETAILS

Clone: MEM-154

Specificity: CD16

Reactivity: Human

Format: FITC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Gessner JE, et al. 1995. J Biol Chem 270:1350-61.
2. Kocher M, et al. 1997. J Immunol 159:3940-8.
3. Arase N, et al. 2003. J Immunol 170:3054-8.
4. Gasdaska JR, et al. 2012. Mol Immunol 50:134-41.
5. Ohradanova-Repic A, et al. 2018. Front Immunol 9:852.
6. Tamm A, et al. 1996. J Immunol 157:1576-81.
7. de Haas M, et al. 1996. J Immunol 156:2948-55.
8. Koene HR, et al. 1997. Blood 90:1109-14.

For research use only. Not intended for use in diagnostic procedures.