The antibody LNK16 reacts with an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

PRODUCT DETAILS

Clone: LNK16

Specificity: CD16

Reactivity: Human, Non-Human Primate

Format: FITC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Gessner JE, et al. 1995. J Biol Chem 270:1350-61.
2. Kocher M, et al. 1997. J Immunol 159:3940-8.
3. Arase N, et al. 2003. J Immunol 170:3054-8.
4. Tamm A, et al. 1996. J Immunol 157:1576-81.
5. Boyle JJ. 2004. Clin Exp Immunol 137:529-37.
6. Lin CW, et al. 2005. Blood 106:3567-74.
7. Mathison RD, et al. 2003. BMC Immunol 4:3.
8. Hovden AO, et al. 2011. BMC Immunol 12:2.

For research use only. Not intended for use in diagnostic procedures.