The mouse monoclonal antibody clone BU15 binds to human CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin α-receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g., fibrinogen (cell-matrix). In addition, integrin α's are active signaling receptors that recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin α-receptors consist of distinct combinations of integrin α-chains, which are differentially expressed. Integrin alpha X (CD11c) assembles with integrin beta-2 (CD18) into a receptor known as CR4, which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells. CD11c is prominently expressed on tissue macrophages and detected on activated neutrophils, granulocytes, some types of activated T cells, and intestinal intraepithelial lymphocytes (IEL).

PRODUCT DETAILS

Clone: BU15

Specificity: CD11c

Reactivity: Human

Format: FITC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.

For research use only. Not intended for use in diagnostic procedures.