The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

PRODUCT DETAILS

Clone: MEM-174

Specificity: CD11b

Reactivity: Human

Format: FITC

Isotype: Mouse IgG2a

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Hentzen ER, et al. 2000. Blood 95:911-20.
2. Lawrence PK, et al. 2007. Vet Immunol Immunopathol 119:287-98.
3. Akramiene D, et al. 2007. Medicina (Kaunas) 43:597-606.
4. Drbal K, et al. 2007. Int Immunol 19:675-84.
5. Hasan S, et al. 2015. FEBS Lett 589:374-9.

For research use only. Not intended for use in diagnostic procedures.