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Mouse thymocytes were incubated overnight at 37°C in medium containing 1 uM dexamethasone. Cells were harvested and stained with FITC Annexin V (35-6409) and 7-AAD Staining Solution (13-6993).

FITC Annexin V

SKU 35-6409-T025
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Annexin V belongs to the annexin family of calcium-dependent phospholipid binding proteins and has a high affinity for phosphatidylserine (PS). One of the characteristic features of early stage apoptosis is the translocation of PS from the inner to the outer leaflet of the plasma membrane, exposing it to the extracellular environment where it can be detected. Fluorochrome conjugated Annexin V can serve as a sensitive detection probe for this hallmark of early stage apoptotic change.

Annexin V is generally used in combination with a viability dye, such as 7-AAD or propidium iodide (PI), that cannot pass through intact cellular membranes. Translocation of PS occurs prior to loss of membrane integrity, therefore viability dyes would be excluded from the cell. As cells progress through the apoptotic process, membranes are compromised and the viability dyes are able to enter the cell. Using this method, cells that are Annexin V positive and viability dye negative are in early apoptosis. If cells are positive for both Annexin V and the viability dye, they are either undergoing apoptotic death or necrosis. When negative for both markers, cells are viable.

Recent Citations:

Gergen AK, Madsen HJ, Li A, Cheng L, Meng X, Fullerton DA, Pratap A, Weyant MJ. Simvastatin Inhibits Histologic Changes Associated with Gastroduodenal Reflux in a Murine Model. Dig Dis Sci. 2022 Jan 10. doi: 10.1007/s10620-021-07344-0. Epub ahead of print. PMID: 35001242.

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Name FITC Annexin V
Cat. No. 35-6409
Protocol Download Protocol
Format FITC
Application Flow Cytometry
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