FastPROBE™ qPCR Hi-ROX Master Mix
FastPROBE™ qPCR Hi-ROX Master Mix is ready-to-use cocktail for the amplification and quantitation of DNA on real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of ≥ 500 nM. An integral component, the Classic++™ Hot Start Taq DNA Polymerase is compatible with all fluorogenic probes including hydrolysis and displacement probes such as TaqMan®, Scorpions® and Molecular Beacons probes. Novel hot start technology prevents primer dimer formation and non-specific amplification resulting in higher yields and specificity. Advanced buffer chemistry means little to no protocol optimization, regardless of template. FastPROBE qPCR Master Mixes are ideal for both standard and fast-cycling real-time PCR applications, providing improved signal to noise ratios, rapid extension rates for early Ct values, and enhanced sensitivity. Use for low copy number detection, microarray validation, SNP genotyping and multiplexing. Please refer to our Instrument Compatibility Guide to ensure you have the correct ROX concentration for your system.
Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006