Tonbo EZ-BrdU™ Kit
The incorporation of BrdU into newly synthesized DNA by actively cycling cells is one method for measuring the changing amount of cellular DNA during cell proliferation through each of the cell cycle phases. As a thymidine analog, BrdU is preferentially incorporated into newly replicated DNA which can then be subsequently labeled and analyzed to determine relative DNA content and cell cycle position. Incorporation of BrdU is most commonly detected using anti-BrdU antibodies.
A BrdU solution is provided for exposure of actively cycling cells to incorporate BrdU. The EZ-BrdU Kit employs an acid denaturation step. The mild acid method used helps reduce damage to other cellular proteins. After the denaturation step, cells are stained with a FITC anti-BrdU antibody and total DNA is counterstained with a PI/RNase A solution. Two color flow cytometry can then be used to analyze cells that have incorporated BrdU (proliferating cells) in terms of their cell cycle position (G0/1, S, or G2/M phase).
Isotype: Mouse IgG1, κ
Application: Flow Cytometry
Storage: The EZ-BrdU Kit is shipped in one container and consists of two packages plus instruction manual. Upon arrival one should be stored at 2-8°C and the other at -20°C. Do not freeze
- Gratzner HG. 1982. Science. 218: 474-475.
- Dolbeare F, Gratzner HG, Pallavicini MG and Gray JW. 1983. Proc Natl Acad Sci USA. 80: 5573-5577.
- Begg AC, McNally NJ, Shrieve DC and Karchner H. 1985. Cytometry. 6: 620-626.
Falini B, Canino S, Sacchi S, Ciani C, Martinelli MF, Gerdes J, Stein H, Pileri S, Gobbi M, Fagioli M, Minelli O and Flenghi L. 1988. Br J Hematol. 69: 311-320.
Williamson K, Halliday I, Hamilton P, Ruddell J, Varma M, Maxwell P, Crockard A and Rowland B. 1993. Cell Prolif. 26: 115-124.
Li X, Tragano F, Melamed MR and Darzynkiewicz Z. 1994. Int J Oncol. 4: 1157-1161.
These products are for research use only. Not intended for use in diagnostic procedures.
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.