PRODUCT DETAILS

Host: Mouse

Isotype: IgG2a

Clonality: Monoclonal

Clone: 2B5

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 1 mg/mL

Storage: 4°C

Formulation: PBS with no preservative; pH 7.2-7.4

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.

Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.

Applications Tested: ELISA, Flow Cytometry, Western Blotting.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.