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Cytek<sup>®</sup> 20-Color AML Panel, cFluor<sup>®</sup> Reagent Kit (19C)

Cytek® 20-Color AML Panel, cFluor® Reagent Kit (19C)

SKU R7-40009

$3,833.00

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Description

The Cytek® 20-Color AML Panel consists of the Cytek® 20-Color AML Panel, cFluor® Reagent Kit (19C), CD19 Monoclonal Antibody (SJ25C1), Super Bright™ 780, eBioscience™, and ViaDye™ Red Fixable Viability Dye Kit.

The Cytek® 20-Color AML Panel allows for AML testing, including identifying and characterizing normal and aberrant cells, immunophenotypic classification and measurable residual disease evaluation in human fresh and frozen bone marrow samples.

This panel has been optimized and titrated for analyzing human fresh and frozen bone marrow samples on systems equipped with violet, blue, and red lasers.

Products required for the panel but not included:

 

PRODUCT DETAILS

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from Light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

The panel contains the following single vial reagents.

Table 1: Composition of the Cytek® 20-Color AML Panel, cFluor® Reagent Kit (19C) (P/N R7-40009), CD19 Monoclonal Antibody (SJ25C1), Super Bright™ 780, eBioscience™ (P/N 78-0198-42), and ViaDye™ Red Fixable Viability Dye Kit (P/N R7-60008) are available to purchase separately.

 

DATA

Figure 1A. Mononuclear cells

Figure 1B. Myeloid cells

Figure 1. Representative data of healthy donor bone marrow samples. A fresh bone marrow sample collected in a heparin tube was stained with the Cytek® 20-Color AML panel and analyzed on a 5 laser Cytek Aurora™ system. Data was analyzed using the gating schema and plot layout published by Wood et al1 and Soh et al2. Data shown was sequentially gated with time gate, singlet gate and then followed with lymphocyte, monocyte, myeloid and blast gates on CD45 vs. SSC plot. Cells from lymphocyte, monocyte and blast gates were combined as mononuclear cells.

 

Figure 2. Representative data of AML frozen bone marrow samples stained with the Cytek® 20-Color AML Panel. Data was generated using a cryo-preserved AML bone marrow sample. The layout of the data plots is based on those published by Wood et al1 and Soh et al2.

 

Figure 3. Empirical lower limit of quantification (LLOQ) determination of the Cytek® 20-Color AML panel for measurable residual disease (MRD) evaluation. A frozen AML bone marrow sample was spiked into a fresh normal bone marrow sample at different dilutions as shown in the graph and tested in triplicates. Representative data from one replicate is shown. The cancer cells are still detectable at 10-5 dilution.

 

RECOMMENDED USAGE

Human fresh and frozen bone marrow PBMC samples have been tested to validate the performance of this kit. Please refer to the section below to download this kit's staining protocols, data acquisition protocols, experiment templates, and fluorochrome list.

Use appropriate personal protective equipment per product safety data sheet when using this product.

 

REFERENCES

  1. Wood, B. L. (2020). Acute myeloid leukemia minimal residual disease detection: The difference from normal approach. Current Protocols in Cytometry, 93, e73. doi: 10.1002/cpcy.73
  2. Soh, K. T., et al. (2022). Development of a 27-color panel for the detection of measurable residual disease in patients diagnosed with acute myeloid leukemia. Cytometry A, 101, 970-983.
  3. Hedley, B. D., et al. (2021). A multicenter study evaluation of the ClearLLab 10C panels. Cytometry B Clin Cytom. 100, 225-234. 

 

For Research Use Only. Not intended for use in diagnostic procedures.

 

cFluor® V547, cFluor® B515, cFluor® B548, cFluor® BYG610, cFluor® R685, cFluor® R720, and cFluor® R840 are equivalent to CF®405L, CF®488A, CF®514, RPE-CF®596R, CF®660C, CF®700, and APC-CF®790T respectively, manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

cFluor® B690 is a tandem dye made with PerCP. cFluor® BYG610, cFluor® BYG667, cFluor® BYG710, cFluor® BYG750, and cFluor® BYG781 are tandem dyes made with R-PE. cFluor® R780 and cFluor® R840 are tandem dyes made with APC. Caution: Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

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