PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: 2A9-1

Format: PE

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 5 µL (0.125 µg)/test

Concentration: 5 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.

Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2A9-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.