The mouse monoclonal antibody clone IT2.2 binds to human CD86 (B7-2), a costimulatory ligand expressed primarily on antigen-presenting cells including monocytes, dendritic cells, activated B cells, and select subsets of T cells. CD86 is a 70–80 kDa type I transmembrane glycoprotein involved in T-cell activation through engagement of CD28 and CTLA-4. Its expression is dynamically upregulated upon immune activation, making it a key marker for assessing antigen-presenting cell maturation and costimulatory capacity. Clone IT2.2 recognizes an extracellular epitope of CD86 and is validated for flow cytometry to assess activation status in immune monitoring, immuno-oncology, and dendritic-cell assays.

PRODUCT DETAILS

Clone: IT2.2

Specificity: CD86 (B7-2)

Reactivity: Human

Format: cFluor^® V670

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Freeman GJ, Gray GS, Gimmi CD, et al. 1993. Science. 262:909–911.
2. Hathaway WE, Bondurant M, Foy TM, et al. 1994. J Immunol Methods. 170:145–155.
3. Guilliams M, Bruhns P, Saeys Y, et al. 2014. Nat Rev Immunol. 14:571–587.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® V670 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Cytek^® cFluor^® V670 is StarBright^™ Violet 670 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.