cFluor® V670 anti-Human CD20 (2H7)
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Description
The 2H7 antibody reacts with human CD20 that is expressed by pre-B cells, resting and activated B cells, follicular dendritic cells, and a small subset of T cells, but is absent on plasma cells. CD20 is a 33-37-kDa transmembrane protein which is differentially phosphorylated in resting and activated B cells. The CD20 molecule plays a role in regulating B cell activation. The 2H7 antibody is reported to be cross-reactive with several nonhuman primate species including baboon, chimpanzee, cynomolgus, and rhesus monkeys. Please choose the appropriate format for each application.
The antibody was purified by affinity chromatography and conjugated to the fluorophore.
PRODUCT DETAILS
Clone: 2H7
Reactivity: Human
Format: cFluor® V670
Isotype: Mouse IgG2b
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze.
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
EXAMPLE DATA
cFluor® V670 anti-Human CD20 (2H7), (right) vs. Isotype Control (left). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® V670 is StarBright™ Violet 670 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.