The mouse monoclonal antibody clone SJ25C1 antibody binds to human CD19, a 95 kDa glycoprotein that acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1), and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounters. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS, or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells. It is also present on follicular dendritic cells and absent on T cells. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. It is reported that the SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.

PRODUCT DETAILS

Clone: SJ25C1

Specificity: CD19 

Reactivity: Human

Format: cFluor^® V605^*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8
2. Kern Petra S.; et al. 1998. Immunity. 9: 519-530
3. Veillette A. et al. 1988. Cell. 55:301-308.

For research use only. Not intended for use in diagnostic procedures.

^*Cytek^® cFluor^® V605 is StarBright^™ Violet 610 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.

cFluor^® V605 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.