The 3G8 monoclonal antibody binds to IgG receptor III (FcγRIII) that are in two forms: CD16a ((FcγRIIIA) and CD16b ((FcγRIIIB). With 95% sequence similarity, they are a conventional 50-65 kD polypeptide-anchored transmembrane protein and a 48 kD GPI-anchored protein, respectively. CD16a is expressed on NK cells and macrophages while CD16b is expressed on neutrophils1,2. CD16a also plays a crucial role for antibody-dependent cellular cytotoxicity (ADCC) by NK cells3. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  3G8

Reactivity:       Human, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey

Format:              cFluor® V505 

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Wirthmueller U, et al. 1992. J Exp Med. 175:1381
2. Smed-Sörensen A, et al. 2008. Blood. 111:5037
3. Wei H Y, et al, 2016. Sci Rep. 6:34310