cFluor® V475 anti-Human CD4 (RPA-T4)
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Description
The RPA-T4 antibody reacts with human CD4, a 59-kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with major histocompatibility complex (MHC) class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2 domain of MHC class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression and is cross-reactive with chimpanzee CD4. This antibody recognizes a different epitope than the anti-human CD4 antibody clone OKT4, thus does not block binding of CD4 clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).
The antibody was purified by affinity chromatography and conjugated to the fluorophore.
PRODUCT DETAILS
Clone: RPA-T4
Reactivity: Human
Format: cFluor® V475
Isotype: Mouse IgG1
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze.
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
EXAMPLE DATA
cFluor® V475 anti-Human CD4 (RPA-T4), (right) vs. Isotype Control (left). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® V475 is StarBright™ Violet 475 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.