The 2H7 monoclonal antibody binds to human CD20, a 33-37 kDa, four-transmembrane phosphoprotein. CD20 is present on all stages of B cell development except for early pro-B cells or plasma cells to play a role in B-cell activation, proliferation and differentiation¹. In some studies, CD20 is thought to act as a Ca2+ channel in the plasma membrane². It is also present on a T cell subset at low levels as well as some follicular dendritic cells. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  2H7

Reactivity:          Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Squirrel Monkey

Format:                cFluor® V450

Isotype:               Mouse IgG2b, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Tedder TF, et al. 1994. Immunol Today. 15(9):450–4
2. Putney JW Jr. 1999. Proc Natl Acad Sci U S A. 96(26):14669-14671

These products are for research use only. Not intended for use in diagnostic procedures.