The HIB19 monoclonal antibody binds to human CD19, a 95-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. CD19 forms a signal transduction complex with the complement receptor 2 (CD21), a tetraspanin membrane protein, TAPA-1 (CD81), and Lue 13 to function as a dominant signaling component on the surface of B cells^1,2. It is expressed in all phases of B cell development, maturation and differentiation except at the terminal stage of differentiation, lost in plasma cells³. It is also present on follicular dendritic cells and absent on T cells.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: HIB19 

Specificity: CD19 

Reactivity: Human, Chimpanzee, Rhesus

Format: cFluor^® V450

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Wang K, et al. 2012. Exp Hematol Oncol. 10.1186/2162-3619-1-36
2. Bradbury LE, et al. 1993. J Immunol. 151(6):2915-2927.
3. Nadler LM, et al. 1983. J Immunol. 131(1):244-250.

For research use only. Not intended for use in diagnostic procedures.