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cFluor® V450 Anti-Human CD14 (M5E2)

cFluor® V450 Anti-Human CD14 (M5E2)

SKU R7-20004
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The M5E2 monoclonal antibody binds to human CD14, a 53-55-kDa glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that works as a receptor on myeloid cells for ligands such as lipopolysaccharide (LPS)1. It is found that CD14 is a receptor for and binds to complexes of LPS and LBP with high affinity2. It expresses on monocytes and macrophages at high levels. It is also present in some interfollicular dendritic cells and macrophages, reticular dendritic cells and Langerhans cells. The antibody was conjugated to a fluorophore and purified by affinity chromatography.



Clone:                  M5E2

Reactivity:          Human, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-Topped Tamarin, Cow, Cynomolgus, Dog, Pigtailed Macaque, Rhesus, Squirrel Monkey

Format:                cFluor® V450

Isotype:               Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS



Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.



  1. Pugin J, et al. 1998. Infect Immun. 66:1174
  2. Wright SD, et al. 1990. Science. 249:1431



These products are for research use only. Not intended for use in diagnostic procedure.


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