The RA3-6B2 monoclonal antibody binds to the human and mouse CD45 isoform known as CD45R, or B220, a 220-kDa protein tyrosine phosphatase. CD45 is one of the most abundant hematopoietic markers and is expressed on all leukocytes (leukocyte common antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others. The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets, and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
The antibody was purified by affinity chromatography and conjugated to the fluorophore.

PRODUCT DETAILS

Clone: RA3-6B2

Reactivity: Mouse

Format: cFluor^® V435

Isotype: Rat IgG2a

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze.

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS SDS

EXAMPLE DATA

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For Research Use Only. Not intended for use in diagnostic procedures.

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