The SJ25C1 monoclonal antibody binds to human CD19, a 95-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. CD19 forms a signal transduction complex with the complement receptor 2 (CD21), a tetraspanin membrane protein, TAPA-1 (CD81), and Leu 13 to function as a dominant signaling component on the surface of B cells^1,2. It is expressed in all phases of B cell development, maturation and differentiation except at the terminal stage of differentiation, lost in plasma cells³. It is also present on follicular dendritic cells and absent on T cells.

The antibody was purified by affinity chromatography and conjugated with a fluorophore under optimal conditions.

PRODUCT DETAILS

Clone: SJ25C1

Specificity: CD19

Reactivity: Human

Format: cFluor^® V435*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Wang K, et al. 2012. Exp Hematol Oncol. 10.1186/2162-3619-1-36
2. Bradbury LE, et al. 1993. J Immunol. 151(6):2915-2927.
3. Nadler LM, et al. 1983. J Immunol. 131(1):244-250.

For research use only. Not intended for use in diagnostic procedures.

^*Cytek^® cFluor^® V435 is StarBright^™ Violet 440 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by BioRad under an Agreement between Bio-Rad supplied to a company, being subject to certain conditions.