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cFluor® V420 Anti-Human CD52 (HI186)

cFluor® V420 Anti-Human CD52 (HI186)

SKU R7-20032
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The HI186 monoclonal antibody binds to human CD52, a 25-29-kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein. CD52, also known as Cambridge pathology 1 antigen (CAMPATH-1), is expressed in high levels on mature lymphocytes and surface of thymocytes. It is also present on NK cells, macrophages/monocytes, eosinophils, neutrophils and dendritic cells and absent from plasma cells, platelets and erythrocytes1. CD52 is known to be efficient in complement-mediated lysis and antibody-mediated cellular toxicity and are clinically used as a drug target for various diseases, including lymphoma, transplant rejection and autoimmune diseases2. The antibody was conjugated to a fluorophore and purified by affinity chromatography.



Clone:                  HI186

Reactivity:         Human, Cynomolgus, Rhesus

Format:                cFluor® V420

Isotype:               Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS



Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.



  1. Zhao Y, et al. 2017. Inflamm Res. 66(7):571-578
  2. Xia, M Q et al. 1993. The Biochemical journal. 633-40


These products are for research use only. Not intended for use in diagnostic procedures.



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