cFluor® V420 Anti-Human CD52 (HI186)
The HI186 monoclonal antibody binds to human CD52, a 25-29-kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein. CD52, also known as Cambridge pathology 1 antigen (CAMPATH-1), is expressed in high levels on mature lymphocytes and surface of thymocytes. It is also present on NK cells, macrophages/monocytes, eosinophils, neutrophils and dendritic cells and absent from plasma cells, platelets and erythrocytes1. CD52 is known to be efficient in complement-mediated lysis and antibody-mediated cellular toxicity and are clinically used as a drug target for various diseases, including lymphoma, transplant rejection and autoimmune diseases2. The antibody was conjugated to a fluorophore and puriﬁed by afﬁnity chromatography.
Reactivity: Human, Cynomolgus, Rhesus
Format: cFluor® V420
Isotype: Mouse IgG2b, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
- Zhao Y, et al. 2017. Inflamm Res. 66(7):571-578
- Xia, M Q et al. 1993. The Biochemical journal. 633-40
These products are for research use only. Not intended for use in diagnostic procedures.