cFluor™ V420 Anti-Human CD3 (SK7)
The SK7 monoclonal antibody binds to human epsilon chain of the CD3 antigen/T-cell antigen receptor, a 20 to 30-kDa protein complex. This complex contains a CD3γ, a CD3δ, a CD3ζ (CD247), two CD3ε, and a T-cell receptor (αβ or γδ) heterodimer1. CD3 is found on all mature T cells, NK T cells and thymocytes. It play a role in recognizing antigen, activating cytotoxic T cell and T helper cell, and signal transduction2. The antibody was conjugated to a fluorophore and puriﬁed by afﬁnity chromatography.
Reactivity: Human, Chimpanzee
Format: cFluor™ V420
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
- Dong, D., et al. 1981. Nature. 573, 546–552
- Weiss A, et al. 1991. Semin Immunol. (5):313-24
These products are for research use only. Not intended for use in diagnostic procedures.