Cytek cFluor™ TBMNK Kit allow for the identification of lymphocytes, total T cells, helper T cells, cytotoxic T cells, NK T cells, B cells, NK cells, total monocytes, nonclassical and classical monocytes in human peripheral blood mononuclear cells and in whole blood.

CD3 is expressed on all mature T cells, NK T cells, and some thymocytes. CD3, a part of the CD3/T cell receptor complex, plays a role in antigen recognition, signal transduction, and T cell activation.

CD4, is expressed on most thymocytes, a major subset of T cells, and on monocytes/macrophages. Functionally, CD4 is associated with thymic differentiation, in conjunction with MHC class II molecules in antigen recognition and with signal transduction.

CD8 is found on thymocytes, on a subset of T cells and on NK cells. This molecule acts as a co-receptor in MHC class I molecules in antigen recognition, has a role in T cell activation and in thymic differentiation.

CD14 is also known as a high affinity LPS receptor and is highly expressed on monocytes and macrophages. It is also expressed on granulocytes, but at a lower level. In addition, CD14 is found on interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells.

CD16 is expressed on NK cells, monocytes and macrophages in the form of CD16a. Another form of CD16, CD16b is expressed on neutrophils. CD16 engagement of IgG leads to NK cell activation, antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis  

CD19 is expressed in the B cell lineage, from pro-B to blastoid B cells. However, it is absent on plasma cells. It is also expressed on follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation.

CD45 is expressed on all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that is involved in cellular proliferation, differentiation and in regulation of immune cell functions.

CD56, within the hematopoietic system is expressed on NK cells and NKT cells, a subset of T cells. In the nervous system, CD56 is expressed by neurons and plays a role in the homotypic adhesion of neural cells.

PRODUCT DETAILS

Category:            Immunophenotyping

Format:                cFluor conjugated antibodies in individual tubes

Test Dilution:       5 µL per test

Application:        Flow Cytometry

Storage:               -2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA

Data Sheets:       TDS SDS

EXAMPLE DATA

RECOMMENDED USAGE

Human peripheral mononuclear cells (PBMC) or whole blood have been tested to validate the performance of this kit. 

• Download SpectroFlo Experiment Template for PBMCs
• Download SpectroFlo Experiment Template for Whole Blood
• Download cFluor Fluorescent Tag List for SpectroFlo
• Download SpectroFlo Acquisition Protocol
• Download the Sample Preparation Guidelines for Cytek® cFluor® TBMNK Kit, 8 Color

REFERENCES

• Clevers H et al. Ann Rev Immunol. 1988 6, 629 (1988)
• Evans RL et al. Immunol. 78, 544 (1981)
• Cole DK et al. Cell Mol Immunol. 1, 81 (2004)
• Ferrero E et al. J. Immunol. 145, 331 (1990)
• Wirthmueller U et al. J Exp Med. 175, (1992)
• Dennis O et al. J Immunol. 170, 73 (2003)
• Loken MR et al. Cytometry. 11, 453 (1990)
• Poli A et al. Immunology. 126, 458 (2009)

These products are for research use only. Not intended for use in diagnostic procedures.

Cytek dye cFluor R780 and its conjugates were developed, manufactured and commercialized by BioLegend, Inc. under the trademark APC/Fire750™. Fluor conjugated antibody manufactured and supplied by BioLegend Inc.

cFluor™ V547, cFluor™ B515, cFluor™ R668 and cFluor™ R720 are equivalent to CF®405L, CF®488A, CF®647 and CF®700 respectively, manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.