The HK1.4 monoclonal antibody binds to mouse Ly-6C, a 15-17kD GPI-linked cell-surface protein. Ly-6C is expressed on monocyte/macrophage cells, endothelial cells, granulocytes and a subset of lymphocytes. Mice strains with the Ly-6.2 alloantigen have subsets of both Ly-6C positive CD4 and CD8 cells while Ly-6.1 expressing strains have only the CD8 Ly-6C positive subset.

The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone: HK1.4

Specificity: Ly-6C

Reactivity: Mouse

Format: cFluor^® R840^1,2

Isotype: Rat IgG2c, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Havran WL, Lancki DW, Moldwin RL, Dialynas DP, Fitch FW. 1988. J Immunol. 140(4):1034-1042.
2. Jutila MA, Kroese FG, Jutila KL, Stall AM, Fiering S, Herzenberg LA, Berg EL, Butcher EC. 1988. Eur J  Immunol. 18(11):1819-1826.
3. Makaroff LE, Hendricks DW, Niec RE, Fink PJ. 2009. Proc Natl Acad Sci USA. 106(12):4799-804.

For research use only. Not intended for use in diagnostic procedures.

¹cFluor^® R840 is equivalent to APC-CF^®790T manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

²cFluor^® R840 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged
exposure to light or fixatives.