The eBio GL3 monoclonal antibody binds to mouse T cell receptor (TCR) γ/δ . The TCR is a heterodimer consisting either of an α and a β chain (TCR α/β) or of a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, the peripheral lymphoid tissues and the peritoneum. Most γ/δ T cells are CD4^- /CD8^- although some can be CD8⁺. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that T γ/δ⁺ cells also play a principal role in antigen presentation. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:         eBio GL3

Reactivity:    Mouse

Format:          cFluor® R780¹

Isotype:             Armenian Hamster IgG

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE 

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Skarstein K, et al. 1995. Immunology. 81:497-501.
2. Harrison LC, et al. 1996. J Exp Med. 184:2167-2174.
3. Wildner G, et al. 1996. Eur J Immunol. 26:2140-2148.
4. Brandes M, et al. 2005. Science. 309:264-268.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

¹cFluor^® R780 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.