The mouse monoclonal antibody clone 2H7 binds to human CD20. CD20 is a phosphoprotein with a molecular weight of 33-37 kDa and has four transmembrane domains. It is present at all stages of B cell development, except for early pro-B cells and plasma cells, and plays a crucial role in B cell activation, proliferation, and differentiation. In some studies, CD20 is thought to act as a Ca2+ channel in the plasma membrane. It is also present on a T cell subset at low levels and some follicular dendritic cells.

PRODUCT DETAILS

Clone: 2H7

Specificity: CD20

Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus Monkey, Pigtail Monkey, Rhesus Macaque, Squirrel Monkey

Format: cFluor^® R735

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Ledbetter et al. 1986. Hum Immunol. 15: 30-43.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® R735 is equivalent to CF^®710 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.