The monoclonal antibody clone L243 binds to the human HLA-DR antigen, a 36-kDa alpha heavy chain, and a 27-kDa beta light chain heterodimeric cell surface glycoprotein in the transmembrane major histocompatibility complex 2 (MHC II) family. HLA-DR, also known as human leukocyte antigen DR isotype, is present on the surface of antigen-presenting cells, including B cells, dendritic cells, macrophages, monocytes, and activated T cells. MHC class II regulates the immune system by playing a critical role in binding and presenting antigen-derived peptides to peptide-MHC II-specific CD4 T cells. The L243 clone identifies HLA-DR molecules, whether peptide-loaded or unoccupied. It does not exhibit cross-reactivity with HLA-DP and HLA-DQ. Clone L243 interacts with a conformational epitope on HLA-DRα, contingent upon the proper folding of the αβ heterodimer.

PRODUCT DETAILS

Clone: L243

Specificity: HLA-DR

Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus macaque, Dog (Canine), Marmoset, Rhesus, Squirrel, Tamarin monkey

Format: cFluor^® R720*

Isotype: Mouse IgG2a

Test Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.

Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.

Marsik C, et al. 2003. Shock 20:493-6.

Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97-100.

For Research Use Only. Not intended for use in diagnostic procedures.

*cFluor^® R720 is equivalent to CF^®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.