The MEM-63 monoclonal antibody binds to CD58, a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily, also known as lymphocyte function-associated antigen 3 (LFA-3). Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on both antigen presenting cells and target cells enhances T cell recognition via the interaction with CD2 on the T cell surface.

PRODUCT DETAILS

Clone: MEM-63

Specificity: CD58

Reactivity: Human

Format: cFluor^® R720^*

Isotype: Mouse IgG1

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223-252.
2. Dustin ML, et al. 1987. Nature 329:846-848.
3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861-1871.
4. Sanders ME, et al. 1988. J. Immunol. 140:1401-1407.
5. Song S, et al. 2021. Frontiers in Immunology. 12: Article 705140.

For research use only. Not intended for use in diagnostic procedures.

^*cFluor^® R720 is equivalent to CF^®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.