The mouse monoclonal antibody clone HI30 binds to all isoforms of human CD45 antigen (Leukocyte Common Antigen); CD45 is a 180-220 kDa single-chain type I transmembrane protein expressed at a high level on all cells of hematopoietic origin, except erythrocytes platelets and non-hematopoietic cells. It is a protein tyrosine phosphatase with several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and CD45RO. It is reported that clones HI30 and 2D1 anti-human CD45 clones can reciprocally block the binding of each other to CD45.

PRODUCT DETAILS

Clone: HI30

Specificity: CD45

Reactivity: Human, Chimpanzee

Format: cFluor^® R720*

Isotype: Mouse IgG1, k

Test Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

Gadhoum SZ, et al. 2008. Nat Chem Biol. 4:751-757.

Lund-Johansen F, et al. 1992. J Immunol. 148:3221-3229

For Research Use Only. Not intended for use in diagnostic procedures.

*cFluor^® R720 is equivalent to CF^®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.