cFluor™ R720 Anti-Human CD4 (SK3)
The SK3 monoclonal antibody binds to human CD4, a 59-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD4 molecule is expressed predominantly on thymocytes and a subpopulation of mature T-helper lymphocytes. It is also present on monocytes at low levels(1) (3). CD4 plays a role in cell-cell interaction by acting as a co-receptor for MHC class II in antigen recognition. CD4 also has been shown to bind glycoprotein 120 on external envelope of HIV(2). The antibody was conjugated to a fluorophore and purified by affinity chromatography.
Format: cFluor™ R720
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. (Do not freeze)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA.
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
- Evans RL, et al. 1981. Immunol. 78:544
- Arno A et al. 1999. J. Infect. Dis. 180:56
- Muench M, et al. 1997. Blood 89:1364
cFluorTM R720 is equivalent to CF®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.
These products are for research use only and are not intended for diagnostic use.