The 6H6 monoclonal antibody binds to human CD123, a 70-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily, also known as the Interleukin-3 receptor alpha chain. CD123 alone has low affinity to IL-3 and develops high affinity when associated with βc (common chain)¹. The CD123 molecule is expressed predominantly on hematopoietic progenitor cells, macrophages, dendritic cells, basophils, eosinophils, monocytes, megakaryocytes and some B cells^2,3. It is also present in myeloid, lymphoid, and vascular endothelial cells⁴. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  6H6

Reactivity:          Human, Rhesus

Format:              cFluor® R720*

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Broughton SE, et al. 2014. Cell Rep. 8:410
2. Macardle PJ, et al. 1996. Cell Immunol. 168:59
3. Yamada T, et al. 1998. J Allergy Clin Immunol. 101:677
4. Sun Q, et al.1996. Blood. 87:83

These products are for research use only. Not intended for use in diagnostic procedures.

*cFluor™ R720 is equivalent to CF®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.